Publications

Explore scientific publications from collaborative research with academic and industry partners.

  • F. Weber, C. Buschmann, R. Shankar, C. Ramke, R. Klementz, M. Leupold, J. Rupprecht, A. Vetter, K. Teschner, S. Klausing, M. Schmeer, M. Schleef (2024), Single-Use Fermenters for Plasmid and Minicircle DNA Production. Molecular Therapy (Abstract for 27th ASGCT Annual Congress, Baltimore)

    Minicircle,pDNA

  • R. Shankar, N. Schäffer, C. Krüsemann, M. Schmeer, M. Schleef (2024), A High Quality Minicircle Production Scale-Up Success Story: AEX-HPLC In-Process Controls to Guide Upstream Process Modification. Molecular Therapy (Abstract for 27th ASGCT Annual Congress, Baltimore)

    Minicircle

  • R. Shankar, N. Schäffer, C. Krüsemann, M. Schmeer, M. Schleef (2024), Template plasmids for mRNA production – focus on poly(A) elements. (Abstract for 31st ESGCT Annual Congress, Rome& Human Gene Therapy)

    pDNA

  • R. Shankar, M. Radukic, N. Schäffer, M. Schmeer, M. Schleef, K. Müller (2024), Stabilizing ITR elements in AAV transfer plasmids. (Abstract for 31st ESGCT Annual Congress, Rome & Human Gene Therapy)

    AAV,pDNA

  • F. Weber, C. Buschmann, R. Shankar, R. Baier, C. Ramke, R. Klementz, M. Leupold, J. Rupprecht, A. Buve, K. Teschner, S. Klausing, M. Schmeer, M. Schleef (2024), Single-Use Fermentors for Plasmid and Minicircle DNA Production. Bioprocess International

    Minicircle,pDNA,R&D

  • R. Shankar, M. Schmeer, M. Schleef (2024), Producing Plasmid DNA Template for Clinical Grade RNA Vaccine Manufacture. Methods Molecular Biology

    pDNA

  • R. Shankar, N. Schäffer, C. Krüsemann, M. Schmeer, M. Schleef (2023), A High Quality minicircle production scale up success story: AEX-HPLC in-process controls to guide upstream process modification. Human Gene Therapy (Abstract for 30th ESGCT Annual Congress, Brussels)

    Minicircle

  • A. Burgardt, M. Strakeljahn, R. Shankar, M. Wiedemann, M. Schleef (2023), Validation of an analytical capillary gel electrophoresis method for the relative quantification of plasmid DNA topoisoforms. Human Gene Therapy (Abstract for 30th ESGCT Annual Congress, Brussels)

    CGE,pDNA

  • A. Zaharie, T. Luttermann, R. Shankar, M. Schmeer, B. Müller, M. Schleef (2023), Quantitative determination of host cell RNA residues against a DNA background by LC-MS. Human Gene Therapy (Abstract for 30th ESGCT Annual Congress, Brussels)

    R&D

  • M. Radukic, R. Shankar, M. Schmeer, M. Schleef, K. Müller (2023), Production of synthetic AAV genomes from minicircle templates for enhanced gene therapy applications. Human Gene Therapy (Abstract for 30th ESGCT Annual Congress, Brussels)

    Minicircle

  • M. Izco, M. Schleef, M. Schmeer, E. Carlos, G. Verona, L. Alvarez-Erviti (2023), Targeted Extracellular Vesicle Gene Therapy for Modulating Alpha-Synuclein Expression in Gut and Spinal Cord. Pharmaceutics

    Minicircle

  • R. Shankar, N. Schäffer, C. Krüsemann, M. Schmeer, M. Schleef (2022), In-process controls for plasmid isoform characterization. (Abstract for 29th ESGCT Annual Congress, Edinburgh, https://doi.org/10.1089/hum.2022.29225.abstracts and Human Gene Therapy)

    pDNA,R&D

  • D. Lock , R. Monjezi, C. Brandes , S. Bates, S. Lennartz, K. Teppert , L. Gehrke, R. Karasakalidou-Seidt, T. Lukic, M. Schmeer, M. Schleef, N. Werchau, M. Eyrich , M. Assenmacher, A. Kaiser, S. Prommersberger, T. Schaser, M. Hudecek (2022), Automated, scaled, transposon-based production of CAR T cells. Journal for Immuno Therapy of Cancer

    Minicircle

  • S. Prommersberger, R. Monjezi, R. Shankar, M. Schmeer, M. Hudecek, Z. Ivics, M. Schleef (2022), Minicircles for CAR T Cell Production by Sleeping Beauty Transposition: A Technological Overview. Gene Therapy Of Cancer – Methods and Protocols

    Minicircle

  • M. Schmeer, M. Schleef, R. Shankar, D. Kobelt, W. Walther (2022), Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: Quality Control of 20 Years Stored GMP-Grade Plasmid DNA. Gene Therapy Of Cancer – Methods and Protocols

    pDNA,R&D

  • R. Shankar, N. Schäffer, M. Schmeer, J.M. Risse, K. Friehs, M. Schleef (2021), Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification. Microbial Cell Factories

    pDNA,R&D

  • M. Izco, J. Blesa, M. Schleef, M. Schmeer, R. Porcari, R Al-Shawi, S. Ellmerich, M. de Toro, C. Gardiner, Y. Seow, A. Reinares.Sebastian, R. Forcen, JP. Simons, V. Belotti, JM. Cooper, L. Alvarez-Erviti (2019), Systemic Exosomal Delivery of shRNA Minicircles Prevents Parkinsonian Pathology. Molecular Therapy

    Minicircle

  • T. Buchholz, M. Schleef (2018), Classification and Regulation of Advanced Therapy Medicinal Products. International Biopharmaceutical Industry

    pDNA

  • T. Buchholz, C.J. Christel, H. Besir (2018), Customised Viral Engineering – Proactive Progress. European Biopharmaceutical Review

    pDNA

  • M. Holstein, C. Mesa-Nuñez, C. Miskey, E. Almarza, V. Poletti, M. Schmeer, E. Grueso, JC. Ordóñez Flores, D. Kobelt, W. Walther, MK. Aneja, J. Geiger, HB Bonig, Z. Izsvák, M. Schleef, C. Rudolph, F. Mavilio, JA. Bueren, G. Guenechea, Z. Ivics (2018), Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors. Molecular Therapy

    Minicircle

  • T. Buchholz, M. Schmeer, M. Schleef (2017), Size Matters. European Biopharmaceutical Review

    Minicircle

  • M. Schmeer, T. Buchholz, M. Schleef (2017), Plasmid DNA Manufacturing for Indirect and Direct Clinical Applications. Human Gene Therapy

    pDNA

  • W. Garrels, TR. Talluri, M. Ziegler, I. Most, DO. Forcato, M. Schmeer, M. Schleef, Z. Ivics, W. A. Kues (2016), Cytoplasmic injection of murine zygotes with Sleeping Beauty transposon plasmids and minicircles results in the efficient generation of germline transgenic mice. Biotechnology Journal

    Minicircle

  • M. Schnödt, M. Schmeer, B. Kracher, C. Krüsemann, L. E. Espinosa, A. Grünert, T. Fuchsluger, A. Rischmüller, M. Schleef, H. Büning (2016), DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations. Molecular Therapy—Nucleic Acids

    AAV,Minicircle

  • R. Monjezi, C. Miskey, T. Gogishvili, M. Schleef, M. Schmeer, H. Einsele, Z. Ivics, M. Hudecek (2016), Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors. Leukemia

    Minicircle

  • I. Freudenau, P. Lutter, R. Baier, M.Schleef, H. Bednarz, A. R. Lara, K. Niehaus (2015), ColE1-plasmid production in Escherichia coli: mathematical simulation and experimental validation. Frontiers in Bioengineering and Biotechnology

    pDNA,R&D

  • H. Akita, D. Kurihara, M.Schmeer, M. Schleef, H. Harashima (2015), Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells. Pharmaceutics

    Minicircle

  • M. Schmeer, M. Schleef (2014), Pharmaceutical Grade Large-Scale Plasmid DNA Manufacturing Process. Methods in Molecular Biology

    pDNA

  • D. Kolbelt, M. Schleef, M. Schmeer, J. Aumann, P.M. Schlag, W. Walther (2013), Performance of High Quality Minicircle DNA for In Vitro and In Vivo Gene Transfer. Molecular Biotechnology

    Minicircle

  • S. Chabot, J. Orio, M. Schmeer, M. Schleef, M. Golzio, J. Teissié (2013), Minicircle DNA Electrotransfer for efficient Tissue targeted Gene Delivery. Gene Therapy

    Minicircle

  • M. Viefhues, S. Wegener, A. Rischmüller, M. Schleef, D. Anselmetti (2013), Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines. Lab Chip

    R&D

  • M. Schleef (2013), Non Viral DNA Vectors. Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology

    Minicircle

  • M. Schleef (2013), Production and Purification of Plasmid Vectors. Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology

    pDNA

  • W. Walther, M. Schmeer, D. Kobelt, R. Baier, A. Harder, V. Walhorn, D. Anselmetti, J. Aumann, I. Fichtner, M. Schleef (2013), A seven-Year Storage Report of Good Manufacturing Practice-Grade Naked Plasmid DNA: Stability, Topology, and In Vitro/In Vivo Functional Analysis. Human Gene Therapy Clinical Development

    pDNA

  • M. Schleef (ed.) (2013), Minicircle and Miniplasmid DNA Vectors, The Future of Non-Viral and Viral Gene Transfer. Wiley-VCH Weinheim

    Minicircle

  • M. Schleef, M. Blaesen, M. Schmeer, R. Baier, C. Marie, G. Gickson, D. Scherman (2010), Production of non viral DNA Vectors. Current Gene Therapy

    Minicircle,pDNA

  • M. Schmeer (2009), Electroporative Gene Transfer. in Molecular Biology

    pDNA

  • M. Schleef, M. Blaesen (2009), Production of Plasmid DNA as a Phamaceutical. Methods in Molecular Biology

    pDNA

  • C. Maucksch, A. Bohla, F. Hoffmann, M. Schleef, M. K. Aneja, M. Elfinger, D. Hartl, C. Rudolph (2009), Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells. The Journal of Gene Medicine

    pDNA,R&D

  • M. Schleef, R. Baier, W. Walther, M.L. Michel, M. Schmeer (2006), Long-Term Stability Study and Topology Analysis of Plasmid DNA By Capillary Gel Electrophoresis. BioProcess International

    pDNA

  • M. Schleef (ed.) (2005), DNA-Pharmaceuticals. Wiley-VCH Weinheim

    pDNA