CGE Service

[Capillary Gel Electrophoresis]

A powerful, fast and reliable analytical separation technique to evaluate DNA topologies

plasmid-application

Capillary Gel Electrophoresis (CGE) is an advanced analytical technique designed to separate and characterize biomolecules based on their size and conformation. Especially in the field of plasmid DNA analysis, CGE offers unmatched precision in resolving different plasmid forms. At its core, CGE utilizes narrow capillaries filled with a gel-like polymer matrix and applies a high-voltage electric field to enable rapid, high-resolution separation of plasmid species. Compared to conventional gel electrophoresis, CGE is faster, more sensitive, and highly reproducible – making it a preferred method in quality control and structural characterization of plasmid DNA.

At PlasmidFactory, we have taken CGE a significant step further. Our proprietary CGE method has been refined over many years of experience and is tailored specifically for the analysis of plasmid DNA, setting a new benchmark for precision and consistency. Unlike generic CGE systems, our technology is finely optimized for plasmid topological assessment and allows us to distinguish and quantify subtle conformational differences with extraordinary accuracy. This makes our CGE Service one of the most reliable and advanced solutions currently available for detailed plasmid quality evaluation.

Capillary gel electropherogram and different types of plasmid topology:

A – B: ccc-forms (monomer and dimer)
C – D: linear-forms (monomer and dimer)
‘E – F: oc-forms (monomer and dimer)

CGE

Flexible CGE Analysis for Plasmid DNA Characterization

We offer CGE analysis as a standalone service or integrated into our contract manufacturing workflows, providing flexibility based on your project needs. Whether you produce plasmid DNA in-house or use our custom manufacturing services, CGE delivers the critical insights required to move your project forward.

Our proprietary CGE platform enables accurate identification and quantification of key plasmid topologies:

  • ccc-monomer (covalently closed circular monomer)
  • ccc-dimer (covalently closed circular dimer)
  • oc-forms (open circular)

These variants are essential quality attributes, particularly in therapeutic applications, gene therapy, and advanced research, where unwanted forms can impact performance, transfection efficiency, and regulatory compliance.

CGE analysis is a standard component of our quality control for CCC grade and all HQ plasmid products, and can be added to Research grade plasmids upon request—allowing you to align analytical depth with your project requirements and budget.

Advanced CGE Technology Supporting Quality, Compliance, and Stability

Our in-house CGE expertise, built on thousands of runs, delivers exceptional resolution beyond standard commercial systems and supports high-confidence analytical data, especially in GMP-like and clinical environments.

CGE is also available as part of our Storage & Logistics Service to monitor long-term plasmid stability during storage and transport. Regular analysis ensures your material remains within defined quality specifications, strengthening reliability in project planning and regulatory documentation.

With PlasmidFactory’s proprietary CGE technology, you gain access to a powerful analytical tool for verifying, validating, and optimizing plasmid DNA quality across research, production, and clinical workflows.

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