Order
You have decided to save time and to reduce your workload: You engage PlasmidFactory to produce your plasmids. Key details such as order quantity and plasmid DNA quality grade, buffer system, required concentration, etc. are clarified.
Sample shipment
You send a 20 µg DNA sample in a padded envelope to PlasmidFactory.
Reception QC
PlasmidFactory blinds your order by assigning a “PF-number” and creates a new database entry, in which all other steps are documented. PlasmidFactory performs the routine reception Quality Control (QC).
Transformation/strain selection
PlasmidFactory transforms E. coli production strains with your plasmid and selects the optimal clone for production. Based on this and depending on the requested Quality grade, a glycerol stock or a comprehensively characterized cell bank is produced.
Cultivation
Cultivation is initiated based on the glycerol stock. PlasmidFactory uses several specific proprietary E. coli production strains.
DNA purification
PlasmidFactory uses optimized and multi-stage chromatographic methods for plasmid purification. This guarantees consistently high DNA quality and reproducibility, ensuring consistency of your research findings. Depending on your individual order, further purification steps may be included to ensure plasmid purity at the highest level.
Product QC
PlasmidFactory prepares a detailed QC report, which you will receive after the production. Up to seven different quality control tests are performed by default and are already included in the service. Further QC tests, e.g. sequencing, can be requested individually.
Dry ice shipment/delivery
Plasmid or minicircle product is transported on dry ice at the requested concentration and amount.
Re-ordering
For each re-order, PlasmidFactory guarantees the same product specifications as in the initial order, ensuring reproducibility of your experiments.
